Ginkgo Biloba Research Today is a free monthly online journal that collates and summarizes the latest research about Ginkgo Biloba, including details on ginkgo biloba, herbal remedies, side effects, benefits.

Determination of active components of Ginkgo biloba in human urine by capillary high-performance liquid chromatography/mass spectrometry with on-line column-switching purification.

Ding S, Dudley E, Chen L, Plummer S, Tang J, Newton RP, Brenton AG

Mass Spectrometry Research Unit, Grove Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.

Ginkgo biloba is one of the most popular herbal nutritional supplements, with terpene lactones and flavonoids being the two major active components. An on-line purification high-performance liquid chromatography/mass spectrometry (HPLC/MS) method was successfully developed for the quantitative determination of flavonoids and terpene lactones excreted in human urine after ingesting the herbal supplement. Satisfactory separation was obtained using a C(18) capillary column made in-house with sample clean-up and pre-concentration achieved using a C(18) pre-column with column switching. High selectivity and limits of detection of 1-18 ng/mL were achieved using a selected ion monitoring (SIM) scan in negative ion mode; the on-line solid-phase extraction (SPE) recovery of the active components in Ginkgo biloba determined in this study was greater than 75%. Copyright (c) 2006 John Wiley & Sons, Ltd.

Published 9 November 2006 in Rapid Commun Mass Spectrom, 20(24): 3619-3624.
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