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Analysis of prominent flavonoid aglycones by high-performance liquid chromatography using a monolithic type column.

Repollés C, Herrero-Martínez JM, Ràfols C

Departament de Química Analítica, Universitat de Barcelona, Diagonal 647, 08028 Barcelona, Spain.

A fast RP-HPLC method for the simultaneous separation of eleven main flavonoid aglycones in food samples was developed. Flavonoids were divided in three groups according to their hydrophobicity and were resolved by a ternary gradient elution and diode array detection within analysis times less than 15 min. The detection limits of the flavonoids studied were in the range between 0.04 and 0.31 microg/mL, with repeatabilities values between 0.4 and 5%. The method was successfully applied to the analysis of these compounds in complex natural samples, like propolis and Ginkgo biloba.

Published 9 October 2006 in J Chromatogr A, 1131(1): 51-7.
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